2.7. Weight loss, released water, and expressible moisture
Weight loss was determined as described by Nakao et al. (1991). Nham with casing (100 g) was accurately weighted before fermentation using a balance (Model AG 204, Mettler Toledo). During fermentation process, Nham was taken and then reweighed. Difference in weight of Nham before and after fermentation was referred to as weight loss. The percentage of water released from samples was measured immediately upon sample collection according to the method of Nakao et al. (1991). Sample with casing was weighed (A). After removing the sample from the casings, water released on the surface was absorbed using filter paper and sample was weighed (B). The empty casing was weighed (C). The percentage of released water was calculated according to the following equation: