Peptone was produced from soya beans and African locust beans. The produced peptones were
evaluated as component of microbiological media for the growth of some bacteria and compared with
some commercial peptones. Some of the tested bacteria are Escherichia coli, Pseudomonas
aeruginosa, Staphylococcus aureus and Bacillus subtilis. The soya beans and African locust beans
peptones supported the growth of all the tested bacteria favourably well when compared to the
commercial peptones. From the peptone broth cultures at absorbance 540 nm, the best growth was
obtained from Escherichia coli in laboratory-produced soya beans peptone and Staphylococcus aureus
in Bacto peptone (a commercial peptone). This may reduce the cost of peptone in Nigeria.