The inhibitor concentration in liquid hemicellulose stream can be adjusted by changing the conditions (such as temperature, delay (retention time), pH, dry matter content), e.g. the severity factor, in lignocellulose fractionation process step used for (at least partial) hemicellulose liquefaction, such as in การทรีตแบบไฮโดรเธอร์มัล, ออโตไฮโดรไลซิส and/or steam explosion with or without addition of acids. By chancing
the conditions in lignocellulose fractionation, the inhibitory สารประกอบ can be adjusted to result in certain level of inhibitory สารประกอบ in liquid stream containing hemicellulose.
5 Alternatively, the concentration of microbial inhibitors in ประเภทเฮมิเซลลูโลส sugar stream is adjusted by purification of the hemicellulose stream by any known methods, ที่รวมถึง but not limited to adsorption, absorption, filtration, stripping, liming, evaporation, extraction or enzymatic treatment.
10 ตามปกติ, the sugar stream containing mainly ประเภทเฮมิเซลลูโลส sugars (C5-rich stream) derived from the lignocellulose fractionation step resulting in hemicellulose liquefaction (at least partially) contain higher amounts of inhibitory สารประกอบ, such as สารประกอบฟีโนลิก, organic acids (such as acetic acid and formic acid), furfural and/or ไฮดรอกซีเมธิลfurfural than sugar stream (C6-rich stream) from enzymatic
15 การไฮโดรไลซ์ of solid fraction containing cellulose and lignin.
The microbial inhibitor concentration in the sugar stream used in cultivation may be adjusted by mixing the ประเภทเฮมิเซลลูโลส sugar steam (C5-rich stream) and cellulosic sugar stream (C6-rich stream). The mixing of the ประเภทเฮมิเซลลูโลส and cellulosic sugar
20 stream can be done in any proportion to achieve the appropriate concentration of inhibitors in the cultivation that allows the growth of oil producing organisms but prevents or significantly inhibits the growth of contaminating organisms (not capable of efficient oil production).
25 Treatments to liquids derived from lignocellulose pre-treatment can be performed that also result in the increased concentration of inhibitors or increase of concentration of certain inhibitors and removal of other inhibitors which can be advantageous. E.g. evaporation of liquid from pre-treatment containing ประเภทเฮมิเซลลูโลส carbohydrates can result in increased concentration of non-volatile
30 inhibitors (such as สารประกอบฟีโนลิกs) and decreased concentration of volatile inhibitors such as furfural, acetic acid and formic acid. Thus, part of the volatile สารประกอบ such as furfural, may be removed during the concentration of carbohydrates
ตามปกติ, in single cell oil production concentrated sugars are used. In concentration of sugar streams from lignocellulose fractionation and enzymatic การไฮโดรไลซ์, ตามปกติ evaporation is used. Evaporation results in the concentration of non-volatile สารประกอบ, e.g. สารประกอบฟีโนลิก, in the sugar concentrate. ที่ควรใช้คือ,
5 organisms are used in single cell oil production which tolerate high concentrations of สารประกอบฟีโนลิกs.
According to one, preferred รุรุ of the การประดิษฐ์ sugar streams from lignocellulose fractionation containing hemicellulose and cellulose are concentrated
10 prior to be fed to single cell oil production process, but no other purification step is performed. Thus the optimal amount of inhibitors that allows growth of จุลินทรีย์สะสมน้ำมัน but inhibits the growth of contaminating จุลินทรีย์ที่ไม่ใช่แบบสะสมน้ำมัน is achieved by adjusting the conditions in lignocellulose fractionation, by evaporation of lignocellulosic sugar streams and by mixing the
15 hemicellulose sugar rich-stream (CS-syrup) and cellulose sugar rich steam (C6- syrup) in fermentation broth.
The enzymatic treatment has long retention time (ตามปกติ 1 to 3 days) and thus it is prone to microbial contamination causing sugar loss and problems in microbial oil
20 production (aerobic fermentation). According to one, preferred รุรุ of the
การประดิษฐ์, the cellulose+lignin fraction from fractionation step which results in at least partial hemicellulose liquefaction is washed only to such extent that it allows enzymatic treatment but inhibits the growth of contaminating microorganisms in
enzymatic การไฮโดรไลซ์ and thus decrease the sugar losses.
25
The amount of inhibitory สารประกอบ in the solid fraction from lignocellulose fractionation containing cellulose can be adjusted by the extent of washing the solid cellulose fraction prior to enzymatic การไฮโดรไลซ์,