RNA from a clinical sample, the real-time RT-PCR assay took 2 h
and 20 min to provide a result, but the multiplex RT-LAMP assay
was faster, requiring only 13–60 min for amplification of FMDV
specific sequences. The multiplex RT-LAMP reaction was also less
expensive: real-time RT-PCR costs approximately 4 US dollars per
reaction, while the multiplex RT-LAMP used reagents that cost
approximately 1.2 US dollars per reaction. Furthermore, improvement of the extraction step using simpler extraction methods