No Fsp DNA was detected from roots where Fsp was not added
in both trials. In the first trial, no ThzID1-M3 DNA was detected
from any of root samples. Without ThzID1-M3, 3.7 pg Fsp DNA/mg
root was detected from 1 sample at days 3, while 1.1 pg/mg was
detected from 1 sample at day 3 with ThzID1-M3. In the second trial,
0.3 pg ThzID1-M3 DNA/mg root was detected from 1 sample at day
5 where Fsp was not added, whereas no ThzID1-M3 was detected
where Fsp was added. With the added Fsp, 4.6 and 11.2 pg Fsp
DNA/mg root were detected from 1 sample at day 3 and 7, respectively,
while Fsp was not detected when ThzID1-M3 was present.
These results indicated that the addition of ThzID1-M3 resulted in
reduction of Fsp population and its root colonization although the
proliferation of ThzID1-M3 was inhibited by Fsp at the same time.
Beside competition, antibiosis and mycoparasitism, T. harzianum
has been known to induce resistance to pathogens in the host plant.
However, we did not observe an resistance induction and growth
promotion from the pea seedlings with ThzID1-M3, when weights
of roots and upper parts of pea seedlings were monitored as well
as discoloration of the roots