The egg white was detached from the egg manually in aseptic conditions. The EW was dilute using 3-
crease with 0.05M Sodium chloride (NaCl) solution. The pH was set to 4.0 by carefully adding up some drops
of 1N HCL and it was watery with equivalent volume of 40%, 60% ethanol5
. Afterward that the combination
was reserved at room temperature for 3-4 hrs incubation. After the incubation the mixtures were centrifuged at
8000 rpm for 15 minutes at 4°celsious. Then the clear solution was used as crude6
.