The principle of aluminum chloride colorimetric method is that aluminum chloride forms acid stable complexes with the C-4 keto group and either the C-3 or C-5 hydroxyl group of flavones and flavonols. In addition, aluminum chloride forms acid labile complexes with them orthodihydroxylgroups in the A- or B-ring of Flavonoid.Total flavonoid content was calculated as % quercetin