2.3. Detection of PRRSV and TTSuV1
PRRSV RNA was assessed by RT-PCR using a broadly reactive primer pair, AU1/AU2 which amplified a 433-bp fragment of American type PRRSV following the reaction conditions previously described (Mardassi et al., 1994). Identification of TTSuV1 infection was done by PCR as described previously (Zhang et al., 2012).