3.2. HPLC analysis of ginger oleoresin
The HPLC profiles of ginger oleoresin prepared without enzyme
pre-treatment (control) and with cellulase pre-treatment are
shown in Fig. 2. Four major peaks were observed at the retention
times of 11.0, 13.1, 39.7 and 47.3 min. The prominent major peak
at the retention time of 13.1 min showed a mass of 294.15 corresponding
to molecular weight of 6-gingerol (Fig. 3B). The peak with
retention time 11.0 min showed a mass of 301.32 which corresponded
to Na+ addict of 6-paradol (Fig. 3A). Other two major
peaks with retention times of 39.7 and 47.3 min showed masses
of 292.16 and 342.38 respectively (Fig. 3C and D). They could be
identified as proton and sodium adducts of 6-methylshogaol and
of 8-methylshogaol respectivley. Presence of 6-methylshogaol
and 8-methylshogaol were reported earlier in ginger (Chen & Ho,
1986; He, Lian, & Lin, 1998). Identification of the 6-methylshogaol
and 8-methylshogaol in ginger oleoresin by comparison of mass in
the present study conforms to the above literature reports.
Presence of 6-paradol in ginger oleoresin is beneficial as oral administration of (6)-paradol in case of dimethylbenzaanthracene
induced buccal carcinogenesis in hamsters at 30 mg/kg body
weight is reported to result in reduction of tumour formation
3.2. HPLC analysis of ginger oleoresinThe HPLC profiles of ginger oleoresin prepared without enzymepre-treatment (control) and with cellulase pre-treatment areshown in Fig. 2. Four major peaks were observed at the retentiontimes of 11.0, 13.1, 39.7 and 47.3 min. The prominent major peakat the retention time of 13.1 min showed a mass of 294.15 correspondingto molecular weight of 6-gingerol (Fig. 3B). The peak withretention time 11.0 min showed a mass of 301.32 which correspondedto Na+ addict of 6-paradol (Fig. 3A). Other two majorpeaks with retention times of 39.7 and 47.3 min showed massesof 292.16 and 342.38 respectively (Fig. 3C and D). They could beidentified as proton and sodium adducts of 6-methylshogaol andof 8-methylshogaol respectivley. Presence of 6-methylshogaoland 8-methylshogaol were reported earlier in ginger (Chen & Ho,1986; He, Lian, & Lin, 1998). Identification of the 6-methylshogaoland 8-methylshogaol in ginger oleoresin by comparison of mass inthe present study conforms to the above literature reports.Presence of 6-paradol in ginger oleoresin is beneficial as oral administration of (6)-paradol in case of dimethylbenzaanthraceneinduced buccal carcinogenesis in hamsters at 30 mg/kg bodyweight is reported to result in reduction of tumour formation
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