The low yield of the biohydrogen production is the main constraint for its industrialization process. In order to improve its production,medium compositions of the hydrogen fermentation by Klebsiella pneumoniae ECU -15 were optimized through the response surface methodology (RSM).
Effect of the culture media optimization,pH and temperature on the biohydrogen production and the hydrogenase activities by Klebsiella pneumoniae ECU-15
Experimental results
showed that the optimum hydrogen production of 5363.8 ml/L was obtained when the concentration of
glucose, the ammonium sulfate and the trace elements were 35.62 g/L,2.78 g/L and 23.15 ml/L at temperature 37.0 C, pH 6.0.H2 evolving hydrogenase was greatly enhanced by the optimization of the medium
compositions.