This
suspension was incubated at 28 °C for 24 h and was used to inoculate
1 L of FCGM. The inoculated 1-L broth was incubated for up to 24 h at
28 °C in an orbital-shaker incubator set at 200 rpm; when the bacterial
growth reached an absorbance of 0.85 at 550nm, the flask was removed
and placed on a stir plate at room temperature.