The overall objective was to evaluate the use of porcine luteinizing hormone (pLH) for synchronization of ovulation in cyclic gilts
and its effect on reproductive function. In an initial study, four littermate pairs of cyclic gilts were given altrenogest (15 mg/d for 14 d).
Gilts received 500 mg cloprostenol (Day 15), 600 IU equine chorionic gonadotropin (eCG) (Day 16) and either 5 mg pLH or saline
(Control) 80 h after eCG. Blood samples were collected every 4 h, from 8 h before pLH/saline treatment to the end of estrus. Following
estrus detection, transcutaneous real-time ultrasonography and AI, all gilts were slaughtered 6 d after the estimated time of ovulation.
Peak plasma pLH concentrations (during the LH surge), as well as the amplitude of the LH surge, were greater in pLH-treated gilts than
in the control (P = 0.01). However, there were no significant differences between treatments in the timing and duration of estrus, or the
timing of ovulation within the estrous period. In a second study, 45 cyclic gilts received altrenogest for 14–18 d, 600 IU eCG (24 h after
last altrenogest), and 5 mg pLH, 750 IU human chorionic gonadotropin (hCG), or saline, 80 h after eCG. For gilts given pLH or hCG,
the diameter of the largest follicle before the onset of ovulation (mean S.E.M.; 8.1 0.2 and 8.1 0.2 mm, respectively) was
smaller than in control gilts (8.6 0.2 mm, P = 0.05). The pLH and hCG groups ovulated sooner after treatment compared to the
saline-treated group (43.2 2.5, 47.6 2.5 and 59.5 2.5 h, respectively; P < 0.01), with the most synchronous ovulation
(P < 0.01) in pLH-treated gilts. Embryo quality (total cell counts and embryo diameter) was not significantly different among
groups. In conclusion, pLH reliably synchronized ovulation in cyclic gilts without significantly affecting embryo quality.
# 2008 Elsevier Inc. All rights reserved.
Keywo
The overall objective was to evaluate the use of porcine luteinizing hormone (pLH) for synchronization of ovulation in cyclic giltsand its effect on reproductive function. In an initial study, four littermate pairs of cyclic gilts were given altrenogest (15 mg/d for 14 d).Gilts received 500 mg cloprostenol (Day 15), 600 IU equine chorionic gonadotropin (eCG) (Day 16) and either 5 mg pLH or saline(Control) 80 h after eCG. Blood samples were collected every 4 h, from 8 h before pLH/saline treatment to the end of estrus. Followingestrus detection, transcutaneous real-time ultrasonography and AI, all gilts were slaughtered 6 d after the estimated time of ovulation.Peak plasma pLH concentrations (during the LH surge), as well as the amplitude of the LH surge, were greater in pLH-treated gilts thanin the control (P = 0.01). However, there were no significant differences between treatments in the timing and duration of estrus, or thetiming of ovulation within the estrous period. In a second study, 45 cyclic gilts received altrenogest for 14–18 d, 600 IU eCG (24 h afterlast altrenogest), and 5 mg pLH, 750 IU human chorionic gonadotropin (hCG), or saline, 80 h after eCG. For gilts given pLH or hCG,the diameter of the largest follicle before the onset of ovulation (mean S.E.M.; 8.1 0.2 and 8.1 0.2 mm, respectively) wassmaller than in control gilts (8.6 0.2 mm, P = 0.05). The pLH and hCG groups ovulated sooner after treatment compared to theกลุ่มที่ได้รับน้ำเกลือ (43.2 2.5, 47.6 2.5 และ 2.5 h, 59.5 ตามลำดับ P < 0.01), มีการตกไข่กันมากที่สุด(P < 0.01) ในแม่สุกรที่ได้รับ pLH ไม่แตกต่างระหว่างคุณภาพอ่อน (เซลล์ผลรวมจำนวนครั้งและอ่อนเส้นผ่าศูนย์กลาง)กลุ่มนี้ ในการสรุป pLH เชื่อถือตรงไข่ในแม่สุกรทุกรอบโดยไม่กระทบกับคุณภาพตัวอ่อนมาก#2008 Elsevier อิงค์ สงวนลิขสิทธิ์Keywo
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