AbstractIntroduction: Computer vision syndrome (CVS) is a condition in which a personexperiences one or more of eye symptoms as a result of prolonged working on a computer.Objectives: To determine the prevalence of CVS symptoms, knowledge and practicesof computer use in students studying in different universities in Malaysia, and to evaluatethe association of various factors in computer use with the occurrence of symptoms.Material and methods: In a cross sectional, questionnaire survey study, data wascollected in college students regarding the demography, use of spectacles, duration ofdaily continuous use of computer, symptoms of CVS, preventive measures taken to reducethe symptoms, use of radiation filter on the computer screen, and lighting in the room.Results: A total of 795 students, aged between 18 and 25 years, from five universities inMalaysia were surveyed. The prevalence of symptoms of CVS (one or more) was foundto be 89.9%; the most disturbing symptom was headache (19.7%) followed by eye strain(16.4%). Students who used computer for more than 2 hours per day experiencedsignificantly more symptoms of CVS (p=0.0001). Looking at far objects in-between thework was significantly (p=0.0008) associated with less frequency of CVS symptoms.The use of radiation filter on the screen (p=0.6777) did not help in reducing the CVSsymptoms. Conclusion: Ninety percent of university students in Malaysia experiencedsymptoms related to CVS, which was seen more often in those who used computer formore than 2 hours continuously per day.