50 mL of sub-cultured seed cells were inoculated into 500 mL
of medium. However, the cells did not grow in medium made
from FW-HPE. To prevent this inhibition from occurring,
another culture was established with an initial medium that
did not contain FW-HPE, but which was continuously feed
FW-HPE after 24 h. The initial medium contained 10 g/L
dextrose, 3 g/L yeast extract, 3 g/L malt extract, and 5 g/L
peptone. After 24 h, sterilized FW-HPE was fed at a constant
dilution rate of 0.86 d1. The pH was maintained at 8.0 by
automatically adding aliquots of 2N HCl