3. Results and discussion
3.1. Influence of enอironmental parameters:
comparison of recycle batch process using
immobilized L. casei with batch process by free
cells
In an attempt to optimize the use of the recycle
batch reactor system, reported in our previous
study (Senthuran et al., 1997), various parameters
having an influence on lactic acid production,
and in turn on the reactor-productivity and -lifetime,
were analysed. Most of the studies were
performed in synthetic medium in order to evaluate
the effect of individual factors, and comparisons
were made with bioprocess using free L.
casei cells. Each process condition was tested for
at least three consecutive runs of recycle batches,
after which a particular parameter was changed
and the process repeated.