The hydrolyzed samples in 250ml Erlenmeyer flasks were sterilized
at 121o
C for15 min, allowed to cool, aseptically inoculated
with 2 ml of 24 hr old culture of S. cerevisiae and incubated
under anaerobic conditions for 7 days. Harvesting was done by
centrifuge at 5000 rpm for 20 minute.