Pancreas and jejunal digesta samples (around 0.3 g) were homogenized (1:9, mass/vol.) with ice-cold sodium chloride solution (154 mmol/L) using an Ultra-Turrax homogenizer (Tekmar Co., Cincinnati,OH) and then centrifuged at 4450 ×g for 15 min at 4 °C to collect supernatant, which was used for subsequent enzymes activities assay.