where t2 and t1 were respective retention times for CPH and lincomycin, and W2 and W1 were the peak widths at half height. Acquired results were used to evaluate criti-cal operational parameters of the chromatographic sys-tem to ensure adequate resolution and reproducibility im- mediately prior to sample analysis.
Standard calibration curves were prepared with seven calibrators over a concentration range of 15 - 500 μg/mL for CPH. Linear least square regression analysis was then employed to correlate peak areas and drug concentrations