Osmotic treatment for anthocyanin impregnation
For infusion the experiments were carried out as per Bellary et al., 2011. Anthocyanin extract was directly used for infusion. The blanched WMR dices were immersed in the anthocyanin extract for a period of 4 h. The WMR dices were subjected to vacuum and ultrasonication treatment as reported by Bellary and Rastogi, 2014. During infusion initially the blanched samples were subjected to vacuum of 20 kPa for 30 min further during restoration period the samples were subjected to intermittent ultrasonication treatment (5 min on and 25 min off cycle) upto 4 h of period. Later the WMR dices were removed from the extract and blotted with tissue paper to remove external moisture. These pieces were pre-weighed and subjected to candy making through osmotic treatment in respective vessels containing osmotic solutions of varying concentration (30, 40, 50, 60 and 70° Brix of sugar solution containing anthocyanin extract). Ratio of the volume of the sample to that of osmotic solution during impregnation and candy making was maintained at 1:5, in order to confirm that the surrounding solution concentration did not vary significantly during the experiment. At the end of 24 h of immersion time during candy making, the samples were withdrawn and blotted gently with tissue paper to remove adhering osmotic solution and at that time weighed. The samples with high anthocyanin content were further subjected to a series of sucrose concentration for candy making process from 30 to 70° Brix. All the experiments were done in triplicate, and the average value were reported. The flow chart for preparation of WMR candies/rolls is presented in
ทำให้มีโฟเลทสูงขึ้นรักษาการออสโมติกFor infusion the experiments were carried out as per Bellary et al., 2011. Anthocyanin extract was directly used for infusion. The blanched WMR dices were immersed in the anthocyanin extract for a period of 4 h. The WMR dices were subjected to vacuum and ultrasonication treatment as reported by Bellary and Rastogi, 2014. During infusion initially the blanched samples were subjected to vacuum of 20 kPa for 30 min further during restoration period the samples were subjected to intermittent ultrasonication treatment (5 min on and 25 min off cycle) upto 4 h of period. Later the WMR dices were removed from the extract and blotted with tissue paper to remove external moisture. These pieces were pre-weighed and subjected to candy making through osmotic treatment in respective vessels containing osmotic solutions of varying concentration (30, 40, 50, 60 and 70° Brix of sugar solution containing anthocyanin extract). Ratio of the volume of the sample to that of osmotic solution during impregnation and candy making was maintained at 1:5, in order to confirm that the surrounding solution concentration did not vary significantly during the experiment. At the end of 24 h of immersion time during candy making, the samples were withdrawn and blotted gently with tissue paper to remove adhering osmotic solution and at that time weighed. The samples with high anthocyanin content were further subjected to a series of sucrose concentration for candy making process from 30 to 70° Brix. All the experiments were done in triplicate, and the average value were reported. The flow chart for preparation of WMR candies/rolls is presented in
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