Mueller Hinton and Sabouraud medium were used for determination of antimicrobial activity (diffusion method). Biscoumarin derivatives disc diffusion method results were presented as the inhibition zones, given in millimeters (mm).
When using the diffusion method, the test samples were dissolved in 30% dimethyl sulfoxide (DMSO) to obtain a 1000 g/ml stock solution. Bacteria inhibition zones were measured in millimeters at the end of an incubation period of 18 h at 37 °C, and fungi zones after 48 h at 25 °C.