We used the MTT assay to monitor cell viability. The MCF-7 cell line presented a decrease in the cell number only at the highest concentrations of lycopene (2.5-10 μM). When the cells were treated with 10 μM, the reduction reached 20% (Figure 1A). A clear effect was observed on the MDA-MB235 cell line only with higher doses of lycopene, but the decrease was statistically insignificant (Figure 1B). After 48 h of treatment, a substantial decrease in the number of viable cells was observed in the MDA-MB-231 cell line, reaching a 30% reduction compared to untreated cells. (Figure 1C)