Twenty-four hours after slaughter, 50 g of meat sample was sampled
from LTL and minced. The minced sample was freeze-dried for the
determination of protein, moisture and ash contents, as described by
AOAC (2005). Total cholesterol of the meat samples was extracted
after direct saponification with saturated methanolic KOH solution,
according to the procedure ofOrellana et al. (2009). Cholesterol was
analysed using a gas chromatograph (Simadzu 14B), helium as a carrier
gas and 5 alpha cholastane as an internal standard, and was separated
on a capillary column (Supelco SPB TM-5, 30 m×0.5μmfilm thickness),
using a split/splitless injection system (split ratio of 1:10). The injection
port and detector were maintained at 270 and 300 °C, respectively.
Column oven temperature was programmed for 3 min at 240 °C,
followed by an increase of 2 °C for 1 min. Total cholesterol content in
meat was calculated based on the external standard technique, from a
standard curvevs.cholesterol concentration.