Supernatant was removed carefully with a sampler and about
10 cc of autoclaved saline was added to the deposits. Then it
was centrifuged after application of vortex and turbulence.
Thus, DEPC was washed from the deposits. About 300 micro liters
of the final deposits were transferred to a microtube 1.5 and
finally about 50 micro liters of the final deposit was transferred
as a bacterial mass to a microtube 1.5, after it was centrifuged
at 14 000 r/min for 2 min and at the next stage, it was analyzed
by PCR test. Moreover, a number of samples were comparatively
tested with or without DEPC to evaluate the necessity of its
application.