2.2.4.1. Esterification of the samples. Twenty mg of BO samples
were esterified with 14 mL/100 mL of boron trifluoride in methanoland the lipid content separated from the methanol phase after
addition of saturated NaCl solution and hexane. The supernatant
was collected and transferred to a 2 mL aluminum screw-cap
septum glass tube and then saturated with nitrogen atmosphere.