Microalgae as a main feedstock has attracted much attention in recent years but is still not economically feasible due to high algal
culture cost. The objective of this study was to develop a comprehensive eco-friendly technology for cultivating microalgae Platymonas
subcordiformis using aquaculture wastewater as growth medium for biomass and biofuel production. Platymonas subcordiformis was
grown in pretreated flounder aquaculture wastewaters taken from different stages. Each of wastewater contained different levels of
nutrients. The biomass yield of microalgae and associated nitrogen and phosphorous removal were investigated. The results showed
that algal cell density increased 8.9 times than the initial level. Platymonas subcordiformis removed nitrogen and phosphorus from
wastewater with an average removal efficiency of 87%–95% for nitrogen and 98%–99% for phosphorus. It was feasible to couple the
removal of nitrogen and phosphorus from wastewater to algal biomass and biofuel production. However, further studies are required to
make this technologies economically viable for algae biofuel production.