Extraction of antibacterial metabolites
The spore suspensions of ERIH-44 were inoculated on Modified Nutrient Glucose Agar (MNGA) broth media and incubated at 28 °C for 6 days. Culture broth was filtered and collected. The collected broth was extracted (partition chromatography) using ethyl acetate solvent. The solvent was removed by rotary evaporator. The crude extract thus obtained was stored at 4 °C.