A preliminary experiment was conducted to generate RAPD pattern with 12 primers to identify those that would be suitable in the present study to ensure reproducibility of RAPD marker data, the primers generating no or faint (nonreproducible)bands were discarded (OPB.03 , OPB.05,OPC.08 and OPD.09). Eight primers showed clear and good amplification results. Most of them, about 7, generated monomorphic banding pattern for all
samples tested (Fig. 2-a), while only one of the twelve primers (OPD.01) showed polymorphic banding pattern. (Fig. 2-b).
The number of amplified fragments
generated by these primers varied from 3 in OPE.03 to 8 in OPA.01 with an average of 5.38 bands for each primer