This assay is based on the reduction of Mo(VI) toMo(V) by the sample
and the subsequent formation of a green phosphate/Mo(V) complex
at acidic pH [26]. An aliquot of 0.1 ml of sample solution at different
concentrati ons was combined in an Eppendorf tubewith 1ml of reagent
solution (0.6 M sulphuric acid, 28 mM sodium phosphate, and 4 mM
ammonium molybdate). The tubes were capped and incubated at
90 °C for 90 min. After that, each sample was allowed to cool to room
temperature and the absorbance was measured at 695 nm against a
control. The control solution consists of 1 ml of reagent solution and
0.1 ml distilled water. The activity of the hydrolysate was expressed as
α-tocopherol equivalents using the following linear equation: