CONCLUSION
Colchicine concentrations at 5,000–6,000
ppm could affect the width and length of stomata
of physic nut clone FF20SBr-3. Two plants of
physic nut clone FF20SBr-3 showed different
characteristics such as leaf size and leaf shape and
especially infl orescence size, with the male fl owers
bigger than in normal plants. The chromosome
number was found to be 2n = 44 chromosomes
indicating that these plants were tetraploid. The
growth of the tetraploid plant was lower than for a
normal plant and the yield was very low compared
to a normal plant.