Therefore, some researchers conducted a study on the bacterial contamination of fluff which presented these contaminated microorganisms for evaluating the hygienic status in hatchery (Nichols and Leaver, 1967; Chen et al., 2002). However, Magwood (1964a) studied the microbial contamination of the air in hatcheries and compared the results with those obtained from the microbiological examination of fluff. He concluded that both tests measured the level of contamination present in the environment but that air sampling was particularly useful because it demonstrated the wide ranges in microbial count. Furthermore Magwood and Marr (1964) found it necessary to investigate the contamination of the surface inside hatcheries. The efficacy of hatchery sanitation programs may be increased by measuring and comparing the bacterial contamination of the air and the surface inside hatcheries (Magwood, 1964b).