The effective size range of TMV as determined by DLS analysis was between 60–900 nm (Fig. 2a) and the most
frequent events were around 80 nm (Fig. 2b). By NTA the reciprocal values were 70–520 nm and around 120 nm (Fig. 2c), respectively. Electrokinetic (Zeta) potential of TMV, which define the effective charge of objects, was −23.5 ฑ 1.80 mV. TEM and AFM were used to determine the morphology and size of TMV. TMV did not exhibit any characteristic features and were of different sizes and shapes, mostly spheroid (Fig. 2d, e). Base cross-sections of selected particles (Fig. 2f) were within size limits, determined by DLS.