Tempone radical scavenging assay
A fi nal concentration of Tempone in the samples was
0.1 mM. Final concentration of extracts was 0.1 mg/mL.
As previously explained sample with no extract served as a
control. Each preparation was incubated for 20 minutes at
room temperature (20 oC), and then measurements involving
4 minutes scanning time were conducted. EPR measurements
were performed using parameters modulation amplitude, 1 G;
modulation frequency, 100 kHz; microwave power, 10 mW;
time constant, 0.032 s; fi eld centre, 3380 G; scan range, 100G.
All experiments were performed fi ve times and presented as
means ± S.D. The amplitude (A) of the middle peak in the EPR
signal was measured, and antioxidative activity (AA) of each
extract was calculated using Eq. 1.