The MIC of raw CNSL determined for selected rumen bacteria is indicated in Table 4. All bacteria possessing a gram-positive-type cell wall were highly or moderately sensitive to raw CNSL. Thus, raw CNSL exhibited a low MIC (1.56–6.25 μg/mL) toward R. flavefaciens, R. albus, E. ruminantium, and B. fibrisolvens and a moderate MIC (25–50 μg/mL) toward S. bovis
and L. ruminis. In contrast, 4 of the 7 gram-negative bacteria tested (S. dextrinosolvens, R. amylophilus, S. ruminantium, and M. elsdenii) were insensitive to raw