Cloning and sequencing
showed that the amplified DNAs contained
nucleotide sequences corresponding to the
3? terminal region of potyvirus genome,
including a part of the nuclear inclusion b
gene, a complete CP gene, and a 3? non-
coding region (NCR) followed by a poly-A
tail. The nucleotide and deduced amino
sequences of TuMV-L, TuMV-R, RC4, and
YC5 have been filed with the GenBank
and assigned the accession numbers
AF530057, AF530056, AY134473, and
AF530055, respectively. The percent iden-
tities between nucleotide and amino acid
sequences of the CP genes and the 3?
NCRs of the four isolates were compared
with other known potyviral sequences. The
result showed that, except for TuMV iso-
lates, none of the known potyviruses
shared more than 90% nucleotide or amino
acid identities in the regions with those of
YC5 and RC4 (Table 2).