(v/v) for 20 min at 4°C. Homogenate was then centrifuged at 10,000 rpm at 4°C for 30 min using Sorvall, legend Micro21R centrifuge. The supernatant was used for the assay of superoxide dismutase (SOD) activity by the method as reported by S. Marklund and G. Marklund [20]. All calculations were made as per gram fresh weight.