The phenolic profile of six wild growing Marrubium vulgare L. (Lamiaceae) collected from different bioclimatic
zones was investigated. Ethanol, ethyl acetate and residual water fractions were investigated
for their antioxidant activity using DPPH assay. Ethyle acetate (EtOAc) fraction showed the highest
antioxidant capacity with IC50 values ranging from 68.98 to 118.15 g/mL.
Eight phenolic acids and five flavonoids were detected in the ethyle acetate fraction and quantified
by high performance liquid chromatography (HPLC) coupled to a diode array detector (DAD), among
themthe concentrationof quercetin-3-d-O-galactoside (5.84–14.46 mg/g DW) was thehighest.Metabolic
profile, consisting of glycosylated flavonoids and phenolic acids, was very regular with only quantitative
differences and absences limited to minor components. A remarkably significant quantitative variation
for the major components was noted. On the basis of chemical composition, principal component analysis
and cluster analysis, M. vulgare was classified into two groups. Population grouping was not concordant
with bioclimatic appurtenance.