Semen was collected from each group once a week for a period of six weeks during the natural breeding season
(Autumn-February to April). For semen collection with AV, 10 bucks were trained for a period of 4 weeks in the
presence of a doe showing heat before the onset of the study. Only four bucks responded, and were used for AV
semen collection technique. For semen collection with EE, bucks were not trained for semen collection and the
rectal probe of 3 - 5 volts was inserted through the rectum for electric stimulation. Following semen collection with either of the two methods, semen samples were kept in a thermoflask at 37˚C and transported to the laboratory
for evaluation within 1 hour after collection [23]. In the laboratory, semen samples were evaluated macroscopically
for semen volume, pH and sperm concentration and microscopically for sperm motility, morphology
and viability.