To demonstrate the shape of silver nano-particles within the hydrogel network, TEM images shown in Fig. 6, were carried out for both type of hydrogels, namely, hydrogel–Ag nano-particles embedded in the hydrogel and hydrogel–Ag nano-particles prepared by in situ technique. To carry out this analysis, CMC hydrogel containing nano-particles were put in distilled water for 3 days, and then their suspension was sonicated for 3 h. This allows the nano-particles to come out of the swollen hydrogel network as a result of the relaxation of polymeric chain entanglements around the nano-particles.