2.3. Vegetative multiplication and evaluation under salinity
of the H1 plants
About 50 days after the beginning of the salt treatment in the
greenhouse, 1.5–2 cm shoot axilary buds were collected from
18 plants corresponding to the haloconditioning treatments B5
(−1MPa + 10mM NaCl×5 days), D5 (−0.75MPa + 10mM
NaCl×5 days), F5 (−0.5MPa + 10mM NaCl×5 days), C8
(−0.75MPa×8 days), E8 (−0.5MPa×8 days) (H0) and to the
SC population (SC0). The apices were surface sterilized and
rooted in vitro as described by Cano et al. (1998). The rooting
medium contained 1/2 MS mineral salts, 100 mg L−1 myoinositol,
1mg L−1 thiamine–HCl, 0.1 mg L−1 IAA, 10 g L−1
sucrose and 8 g L−1 Difco-Bacto agar. After 17 days in the
rooting medium, the H1 and SC1 plants were transferred to
vermiculite for 20 days in a controlled culture chamber, and
then to a hydroponic system for saline evaluation with 100mM
2.3. Vegetative multiplication and evaluation under salinityof the H1 plantsAbout 50 days after the beginning of the salt treatment in thegreenhouse, 1.5–2 cm shoot axilary buds were collected from18 plants corresponding to the haloconditioning treatments B5(−1MPa + 10mM NaCl×5 days), D5 (−0.75MPa + 10mMNaCl×5 days), F5 (−0.5MPa + 10mM NaCl×5 days), C8(−0.75MPa×8 days), E8 (−0.5MPa×8 days) (H0) and to theSC population (SC0). The apices were surface sterilized androoted in vitro as described by Cano et al. (1998). The rootingmedium contained 1/2 MS mineral salts, 100 mg L−1 myoinositol,1mg L−1 thiamine–HCl, 0.1 mg L−1 IAA, 10 g L−1sucrose and 8 g L−1 Difco-Bacto agar. After 17 days in therooting medium, the H1 and SC1 plants were transferred tovermiculite for 20 days in a controlled culture chamber, andthen to a hydroponic system for saline evaluation with 100mM
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