The biohydrogen production from lignocellulose hydrolysates obtained more attractions since 2006. Previous studies of biohydrogen production, they are normally by using pure cultures in the batch mode. Biohydrogen production by lignocellulosic feedstock hydrolysates is a biological process, which include several parameters, such as different hydrolysis method, substrate source, substrate concentration, operating temperature, pH etc. The feedstock hydrolysates from biomass is quite similar to the process of bioethanol. The performances of biohydrogen production in previous investigations were reviewed, and shown in Table 1 for a batch process and Table 2 for a continuous process. Dark fermentative hydrogen production has received increasing attention in recent years, due to its high hydrogen production rate (HPR). The proposed three-phase fluidized bed bioreactors showed promising H2 producing performance and may have a potential to apply in practical biohydrogen production processes. The fluidized-bed bioreactor holds the advantage of being flexible to operate and easy to scale up and it seems to have the potential to be practically applied in large-scale biohydrogen production from organic wastes. For the sugary wastewater, enrichment in the presence of 2-bromoethanesulfonic acid resulted in lower H2 yields due to increased propionate production and decreased butyrate/acetate ratio. Adding Fe2+ helps improve the H2 production by 105% and speeds up the reaction. Adding l-cysteine enhances the H2 production by nearly 50%. Adding proper concentration of calcium ion could enhance bioH2 producing. Using activated carbon to remove bio-toxic inhibitors can improve the hydrogen yield. The rice straw hydrolysate mixing the feeding substrate with another high strength organic wastewater could enhance the productivity of biohydrogen in the continuous system. Sulfate concentration has a negative effect on biohydrogen production. An analysis of the economic benefits of this innovative reference commercial model prove its feasibility by using beverage wastewater and agricultural waste as feedstock which will enable a hydrogen-producing system to gain a maximum annual profit with an annual return rate of approximately 60% and 39% with Aspen Plus; 81% and 30% with local price evaluation. Some of the example from different sources, performance, and its operating conditions are reviewed and shown in Table 3.