65% monounsaturated extracts have the same overall thickness with or without the sterol, whereas more polyunsaturated membranes are significant thinner in the absence of ergosterol. The amount of AmB insertion in ergosterol-containing membranes is not directly linked to the fraction of sterols, but depends on the degree of lipid polyunsaturation, with most AmB inserted in the relatively mono-unsaturated membranes that contain the least sterols.