[18] were explaining the determination method of fat rancidity
through TBA (thiobarbituric acid) value. The rancidity of fat contained malonaldehyde which determined by
destilation is then to be reacted with thiobarbituric acid. [16] reported that the TBA value of fresh beef ranged from
0.61 to 0.69 mg / kg, whereas [8] reported that freezing meat could decreasing the TBA value. The frozen beef’s
TBA values ranged from 0.27 to 0.41 mg / kg.