Ferric-reducing/antioxidant power (FRAP) was performed according to the method
described by Liu, Qiu, Ding, and Yao (2008) with some modifications. In brief, 50 ml of the supernatant above obtained (diluted if necessary) were mixed with 3 ml of freshly prepared FRAP reagent and the reaction mixtures incubated at 37 C for 30 min. Absorbance at 593 nm was determined against distilled water blank. The FRAP reagent included 0.01 mol/l TPTZ (2, 4, 6-tripyridyl-s-triazine) solution in 0.04 mol/l HCl, 0.02 mol/l FeCl3 solution and 0.3 mol/l acetate buffer (pH3.6) in proportions of 1:1:10(v/v). Aqueous solutions of ferrous sulfate (0e1000 mmol/l) were used for calibration. The tests were performed in triplicate. The results for each cracker and GBF are expressed as mmol of Fe (II)/g oil-free dry basis and mmol of Fe (II)/g dry basis, respectively
Ferric-reducing/antioxidant power (FRAP) was performed according to the methoddescribed by Liu, Qiu, Ding, and Yao (2008) with some modifications. In brief, 50 ml of the supernatant above obtained (diluted if necessary) were mixed with 3 ml of freshly prepared FRAP reagent and the reaction mixtures incubated at 37 C for 30 min. Absorbance at 593 nm was determined against distilled water blank. The FRAP reagent included 0.01 mol/l TPTZ (2, 4, 6-tripyridyl-s-triazine) solution in 0.04 mol/l HCl, 0.02 mol/l FeCl3 solution and 0.3 mol/l acetate buffer (pH3.6) in proportions of 1:1:10(v/v). Aqueous solutions of ferrous sulfate (0e1000 mmol/l) were used for calibration. The tests were performed in triplicate. The results for each cracker and GBF are expressed as mmol of Fe (II)/g oil-free dry basis and mmol of Fe (II)/g dry basis, respectively
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