2.3. Preparation of water-soluble garlic extracts
FRG was cultivated and harvested in Namhae (Korea). All of the garlic pieces
were peeled and crushed. Crushed FRG was suspended with five volumes of DW
(100 g/500 mL). Suspended crushed FRG was extracted for 2 h at different temperatures
(25 C and 95 C for the FRGE and HRGE, respectively) with shaking in a
water bath. The water extracts were filtered twice through four pieces of Cheesecloth
(Kavon Filter Products Co., Farmingdale, NJ, USA), freeze-dried, powdered,
and stored at 20 C until further analysis. At the time of the experiment, the dried
materials were dissolved in DW at the indicated concentrations.
2.3. Preparation of water-soluble garlic extractsFRG was cultivated and harvested in Namhae (Korea). All of the garlic pieceswere peeled and crushed. Crushed FRG was suspended with five volumes of DW(100 g/500 mL). Suspended crushed FRG was extracted for 2 h at different temperatures(25 C and 95 C for the FRGE and HRGE, respectively) with shaking in awater bath. The water extracts were filtered twice through four pieces of Cheesecloth(Kavon Filter Products Co., Farmingdale, NJ, USA), freeze-dried, powdered,and stored at 20 C until further analysis. At the time of the experiment, the driedmaterials were dissolved in DW at the indicated concentrations.
การแปล กรุณารอสักครู่..
