Fungal growth experiments were conducted in desiccator
jars using a novel flow-through system described previously
(Roze et al., 2004). For each treatment, three Petri plates
with inoculated peanuts were placed into one chamber
resulting in triplicate samples. Where no CO2 and/or
ethylene was added, CO2 or ethylene scrubbers (5 g soda
lime for CO2; 5 g KMNO4 for ethylene) were placed in the
growth chamber to minimize effects due to either production
of these gases by the fungus or external contamination
by these gases (Roze et al., 2004). Desiccator jars carrying
inoculated, uninoculated, and no treatment control peanuts
were incubated for 5 days (unless otherwise noted) in
the dark at 30 1C; air/gas mixtures were water saturated by
passing through a sparger containing distilled water. Mold growth was determined by visual inspection.