A new bacterial strain exhibiting laccase activity was isolated from forest soil and was identified as Bacillus
licheniformis LS04. The spore laccase of B. licheniformis LS04 demonstrated a broad pH range for catalyzing
substrates. It was quite stable at high temperature and alkaline pH. There was no loss of
laccase activity after 10 days incubation at pH 9.0, and about 16% of the initial activity was detected after
10 h at 80 C. In addition, the spore laccase was tolerant towards 1 M of NaCl and 30% of organic solvents.
Reactive black 5, reactive blue 19 and indigo carmine were decolorized by the spore laccase in the
absence of mediator. Meanwhile, the decolorization process was efficiently promoted when acetosyringone
was present, with more than 80% of color removal in 1 h at pH 6.6 or 9.0. The unusual properties
indicated a high potential in industrial applications for this novel spore laccase.