According to Melero et al. (2011), mechanical practice to mitigate soil compaction under NT has a negative effect on organic carbon and on enzymatic activities, producing, in short-term, losses in organic matter, reducing soil biochemical quality. Furthermore, the chisel mechanical action might break soil aggregates, decreasing their size similarly to the soil preparation with plow and harrow disk (Fabrizzi et al., 2009), which initially reduces macro-aggregates percentage (Colonego and Rosolem, 2008). Therefore, the mechanical chiseling on soil under NT may have negative effects; decreasing soil organic carbon level and reducing the soil aggregates size and stability.