Nucleic Acids and the Genetic Material Problem Set 1
Problem 6: Meselson-Stahl DNA replication experiment
in the Meselson-Stahl DNA, replication experiment, if the cells were first grown for many generations in 15N containing media, and then switched to AN containing media, what percent of the DNA had 1 light and 1 heavy strand after 2 generations of growth in N growth media? Meselson and Stahl's Experiment Meselson and Stahl in 1957 gave experimental evidence that each DNA strand served as a template for new synthesis, a process called semi-conservative replication E.coli grown in 15N nitrogen (heavy isotope) or three generations Switch to N nitrogen (ight) and after one, two, samples of DNA. heavy and light DNA. Mix with cesium chloride and separate