The rinsed coupons were fixed in 1 M sodium cacodylate buffer containing 2.5% glutaraldehyde and 4% paraformaldehyde at 4 °C for at least 30 min and washed three times in 0.1 M sodium cacodylate buffer for 10 min at 21 ± 2 °C.
The rinsed coupons were fixed in 1 M sodium cacodylatebuffer containing 2.5% glutaraldehyde and 4% paraformaldehyde at4 °C for at least 30 min and washed three times in 0.1 M sodiumcacodylate buffer for 10 min at 21 ± 2 °C.