The isolated bacteria were identified basing on the analysis of
the 16S rRNA gene sequence and using the primers 27F and 1492R
[16]. After a serial dilution in mineral medium, individual colonies
were selected and purified by
repeated streaking on culture medium
containing; mineral medium and
1% of antibiotic solution.
Genomic DNA from pure strains was extracted by sodium dodecyl
sulfate (SDS)-proteinase K treatment